Cyclohexane (cas no 110 82 7) can be used in HPLC, but only in specific situations, mainly in normal-phase chromatography. It is not commonly used in reversed-phase HPLC.
What Is HPLC Solvent?
High-performance liquid chromatography (HPLC) solvents are high-purity liquids used as the mobile phase in HPLC to dissolve samples. HPLC Solvent are characterized by high purity and low viscosity.
Cyclohexane solvent is a suitable, though less common, non-polar HPLC solvent often used in normal-phase chromatography for hydrocarbon/lipid separations. It is a strong alternative to n-hexane, offering similar UV-cutoff (~200–220 nm) but higher safety.
What Is Cyclohexane Solvent (cas no 110 82 7) ?
Cyclohexane (cas no 110 82 7) is a colorless, flammable liquid with a detergent-like odor. As a non-polar solvent, it is widely used in numerous industrial fields. It is suitable for high-performance liquid chromatography (HPLC), capable of dissolving a wide variety of organic compounds, making it an indispensable solvent in analytical and preparative chemistry. Its low viscosity and high purity ensure the stability of HPLC systems, thereby guaranteeing the accuracy and repeatability of chemical testing and analysis results.
Beyond chromatographic applications, cyclohexane solvent is also a key precursor in the production of nylon and other synthetic fibers, demonstrating its versatility in the chemical industry.
Key Properties of Cyclohexane
- Chemical formula: C₆H₁₂
- CAS number: 110-82-7
- Appearance: Clear, colorless liquid
- Odor: Mild, gasoline-like smell
- Boiling point: Approximately 80.7°C
- Solubility: Insoluble in water, soluble in organic solvents
- Flammability: Highly flammable
For more information on Liquid Cyclohexane Specification, please contact us.
Advantages of Using Cyclohexane in HPLC
| Property | Benefit |
|---|---|
| Low polarity | Ideal for normal-phase separations |
| Low UV absorbance | Suitable for UV detection at low wavelengths |
| Good solvency | Dissolves non-polar analytes effectively |
| Volatile | Easy to remove after analysis |
Limitations:
- High Melting Point: Cyclohexane solvent freezes at 6.5°C, which means it can solidify in the column or fittings if the lab is cold or if it is stored in a refrigerator.
- Pressure Limitations: It can become solid at high pressures (above ~200 bar), which can break pumps.
- UV Cut-off: It has a low UV cut-off, generally around 200-220 nm, making it useful for UV detection, but not as low as some other solvents.
- Viscosity/Polarity: It has low viscosity, ensuring good flow rates, and low polarity
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Cyclohexane solvent other uses
CAS 110-82-7 Cyclohexane is a cycloalkane compound commonly used as an organic solvent and as a precursor in nylon production. The majority of cyclohexane produced is consumed in the production of cyclohecanol and cyclohexanone. These compounds are then used to make adipic acid and caprolactam which are major components in the manufacturing of nylon 6 and nylon 6,6.
about us
As a leading manufacturer and exporter of fine chemicals, Gneebio provides high-purity Cyclohexane Organic Solvent that meets both industrial and laboratory-grade standards. We supply to customers across the chemical, pharmaceutical, and materials industries, ensuring consistent quality, reliable logistics, and professional technical support.
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Gneebio Product Packaging
| Packaging Type | Net Weight |
|---|---|
| Steel Drum | 250 kg |
| IBC Tank | 1250 kg |
| ISO Tank | Bulk shipment |
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